One condition equivalent to integrability of the almost complex structure is ∂2=ˉ∂2=0. If we're just talking about quantum mechanics with a Kähler target, then the Hilbert space is the space of complex-valued differential forms on the target with integration against the symplectic volume form giving the Hilbert space pairing. Then some combination of the supercharges act as ∂ and some as ˉ∂. The N=2 algebra relations imply the integrability condition above.
I think that for a symplectic target, while it is possible to define the A-model, it is not a topological twist of a well-defined N=2 sigma model.