Write f(x0,E,δ) for the integral, taken between x0+δ and x1−δ, where δ>0 is tiny. Then you may differentiate under the integral with respect to E to get dfdE(x0,E,δ). Then you must show that the limit of the result remains well-defined when δ→0 to get dfdE(x0,E,0). Now you may use the chain rule to calculate the derivative of T(x0)=f(x0,U(x0),0).